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KMID : 0363320030240020348
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Journal of Korean Oriental Internal Medicine
2003 Volume.24 No. 2 p.348 ~ p.357
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The Effect of Aucklandiae Radix.Moschus(ÙÊúÅ.ÞúúÅ)¡¯s for Delayed Neuronal Death in Hypoxia
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Ryu Do-Kyun
Jeong Seung-Hyun
Shin Gil-Cho
Lee Won-Chul
Moon Il-Soo
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Abstract
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Objectives : The purpose of this investigation is to evaluate the effects of Aucklandiae Radix Moschus(ÙÊúÅ ÞúúÅ)and to study the mechanism for neuronal death protection in hypoxia with Embryonic day 20 (E20) cortical cells of a rat (Sprague Dawley).
Methods : E20 cortical cells used in this investigation were dissociated in Neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, Aucklandiae Radix Moschus(ÙÊúÅ ÞúúÅ) was added to the culture media for 72 hrs. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, Moschus(ÞúúÅ)¡¯s effects for neuronal death protection were evaluated by LDH assay and the mechanisms were studied by Bcl-2, Bak, Bax, caspase family.
Results : This study indicate that Aucklandiae Radix(ÙÊúÅ)¡¯s effects for neuronal death protection in normoxia and Scutellariae Radix(ÞúúÅ)¡¯s effects for neuronal death protection in hypoxia were confirmed by LDH assay in culture method of Embryonic day 20(E20) cortical neuroblast. Moschus(ÞúúÅ)¡¯s mechanism for neuronal death protection in hypoxia is to increase the anti-apoptosis protein Bcl-2.
Conclusions : It may be reasonable to propose that Moschus(ÞúúÅ) protects delayed neuronal death in hypoxia by increasing Bcl-2, thereby reducing mitochondrial permeability transition(PT) pores, the cytochrome c channels.
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KEYWORD
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Radix, Moschus, Hypoxia, Bcl-2
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